Custom Protein Expression

General Biosystems offers professional protein expression and purification service in most host systems. Combined with our maximum or soluble expression service, we can meet most of your protein production needs.

Service Descriptions

Service available for most commonly used hosts, including Escherichia coli, yeast and mammalian cells. If you want to use other host cells or cell-free expression system, please contact us.
A variety of expression labels are available based on customer needs, including His, Sumo, GST, MBP, etc.
Expression optimization is specific to the expression vector that customer uses.

Service Procedure

Multiple Protein expression system 

Expression System	System Benefits	Application	Features of Generalbiosystems
E. coli Protein  Expression System	High target gene expression quantity, low cost, simple culture conditions, product rapidly, strong scalability, simple conversion operation, easy to form disulfide bond	Prokaryotic proteins, simple eukaryotic proteins	Expression includes soluble protein, inclusion body, fusion proteins, etc., with wealthy experience and expertise, we can solve a variety of bottlenecks during the protein expression process
Yeast Protein Expression System	Cost-effective, low-cost for amplifying medium, simple culture conditions, production rapidly, strong scalability, good choice for secretory protein or intracellular protein expression, secrete proteins efficiently and allow simple purification, extensive post-translational modifications, no endotoxin	Industrial strain improvement, amplification	The combination of self-transformed efficient secretion vector and host can achieve the highest quality protein expression to the maximum extent; Patented Biobrick technology can be successfully used to the improvement and optimization of industrial strain
Insect baculovirus Protein  expression system	Large gene capacity, high efficiency of exogenous gene expression, effective cell fold, moderate scalability, extensive post-translational modifications, glycosylation similar to mammalian cells, is relatively easy enzymatic deglycosylation, no endotoxin	Virus vaccines, signal proteins, cytokines, kinases, etc.	Adopt AcNPV-sf9 cells and high5 cells two expression systems, the selectivity of multiple expression systems, multiple hosts, multi-carrier greatly improve the success rate of protein expression
Mammalian Cell Protein  Expression System	Higher expression levels, moderate scalability, cell suspension culture characteristic can do mass production, effective protein fold, suitable for protein secretion, full post-translational modifications, no endotoxin	Complex higher eukaryotes proteins	Adopt the specific combined methods of mammalian cell expression vector and a variety of transfection, optimize expression conditions, improve transfection efficiency, greatly shorten the experimental period, significantly increase the expression quantity

Service Options

Online Order

Download and fill out the order form and email to  info@generalbiosystems.com. We will promptly confirm the order with you and arrange for production.

Order form Dowload to ensurea successful upload of your order,pls download the forms

Email:info@generalbiosystems.com

Phone:888-778-6481