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Customized protein expression and purification services

Update time : 2020-10-21

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Customized protein expression and purification services

With the development of biopharmaceuticals and recombinant protein expression technology widely used in research fields such as life sciences and medicine, the demand for low-cost, short-period, and high-purity active proteins is also increasing. Conventional host cells used for recombinant protein expression are usually derived from expression systems such as prokaryotic cells, yeast cells, baculovirus, E. coli, insect cells, and mammalian cells.

 

Based on a complete synthetic biology platform, with the help of industry-leading codon optimization software and more than ten years of professional protein expression and purification experience, General Biosystems provides scientists all over the world with customized protein expression services of milligrams to grams of high purity activity.  We focus on high-quality protein production, which can effectively save your economic and time costs.

 

Customized protein expression service content:

Codon optimization: free optimization of patented software, leading optimization parameter settings

Customized expression of prokaryotic proteins: leading sequence optimization and assembly platform, free choice of thousands of vectors

Guaranteed expression of prokaryotic proteins: codon optimization technology combined with patented vectors guarantees expression success rate >95%

Yeast protein custom expression: eukaryotic expression system, which can post-translationally modify most proteins

Baculovirus-insect cell expression: suitable for large fragments and toxic protein expression, the production scale can reach 500 L

Mammalian cell expression: proprietary signal peptide library, optimized expression conditions, production of recombinant protein from milligram to gram


Protein expression system selection reference:

Escherichia coli expression system

Yeast Expression System

Baculovirus-insect cell expression system

Mammalian cell expression system

Advantage

The production time is short, the cost is low, and it is easy to scale up production; the genetic background of the strain is clear.

The production time is short; the eukaryotic expression system can post-translationally modify most proteins.

Suitable for expressing large fragments of DNA and some toxic proteins;

Folding and post-translational modification of most proteins

All proteins can be folded and the most natural post-translational modification

Disadvantage

No post-translational modification; easy to form inclusion bodies.

The protease secreted by yeast itself may degrade the target protein;

Glycosylation is different from mammalian cells

High cost; difficult to scale up production.

high cost

Recommended use

Antigen; protein standard; functional research; structural research;

vaccine;

Glycosylation research;

Secreted protein expression;

Glycosylation research;

Activity analysis;

Secreted protein expression;

Functional research

Structural research

Activity analysis;

Secreted protein expression;

Protein expression service order

Email: Please send your detailed requirements to info@generalbiosystems.com

Telephone order: You can directly call the toll-free hotline 888-778-6481 to contact our experienced engineers