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Gene synthesis method

Update time : 2020-08-19

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Gene synthesis method

The methods of gene synthesis cannot be substituted for each other, and each method can be applied in different experiments. 

Here is an overview of several common gene synthesis methods:

Solid phase synthesis

Traditional oligonucleotide synthesis uses a microliter volume of solution for synthesis on a small column. 

Oligonucleotide synthesis involves adding nucleotide monomers one by one at the end of the synthetic chain. 

The synthesis of each oligonucleotide contains four steps: deprotection, coupling, blocking and oxidation. 

Sequence integrity and synthetic productivity hinder product lengths longer than 200bp, so this method generally limits the length of DNA sequences. 

The main advantage of this method is its high accuracy, which also makes people accept the high cost and low output of this method.

Chip DNA synthesis

As the name suggests, chip DNA synthesis is a technology that uses a series of electrochemical techniques to synthesize DNA using a microarray chip. 

Different kinds of nucleotides can be synthesized at different specific positions on the chip, which are called oligonucleotide pools. Following this segmented synthesis method, gene fragments are amplified in the oligo-nucleic acid pool, and then aggregated and assembled into the finished product. 

The chip DNA synthesis method is cheaper than solid-phase synthesis and can produce a larger number of target genes, but the accuracy of this method is comparable.

PCR synthesis

The gene fragments generated by the PCR method can be completed by various cell systems. For example, using the yeast system, different genes can be added to the yeast chromosomes by using different nick labeling enzymes and tags. 

Since it has the characteristics of being able to insert genes into the target genome, there is no length limit to the extent that the chromosome can accommodate. 

This method has a good effect when synthesizing long gene fragments. With the help of the cell system, the accuracy of the gene sequence can be guaranteed.

Gene synthesis applications

Gene synthesis has a wide range of applications in many fields, including more aspects from the gene loop to the improvement of metabolism. 

With the advancement of our technology, our team's understanding of gene synthesis deepens, and scientists can modify and design genes. 

Researchers have been able to synthesize and assemble a gene fragment with a gene length of more than 100kbp. When it is inserted into a host bacteria lacking its own genetic material, the bacteria can successfully produce new cells.

Generalbiosystems' gene synthesis platform uses patented cloning technology to help customers clone target genes at any designated position on the desired vector without relying on vector restriction sites to meet customers' various cloning design plans.