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Use of PCR

Update time : 2020-08-31

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Use of PCR
PCR can be very effective for modifying DNA. Such modifications may include the addition of restriction enzyme sites (to facilitate cloning requirements) or regulatory elements (for example, the addition of a promoter sequence to a DNA cistron).
Another type of modification can be the generation of targeted mutations at the desired site in the gene, including sequence changes, additions or deletions.

Cycle sequencing is Fred Sanger's first improvement to the classic dideoxy sequencing method in the early 1980s. It uses PCR principles to quickly perform sequence reactions in a thermal cycler. 

For PCR-guided diagnosis, for example, coarse samples and small amounts of substances can be processed, which may include degraded templates, blood, sperm, tissue, individual hair, etc.

With the development of molecular biology, the analysis of pathogen genetic material has supplemented or replaced serological methods for diagnosis, epidemiology and taxonomy. The ability to indicate the presence of pathogens by detecting their DNA or RNA has a significant advantage.

PCR represents a completely new technology. In vitro bacteria or virus cultures are widely used to isolate and propagate pathogens. Therefore, because of their large presence and few pollutants, the organism itself or its antigens can be detected more easily. PCR technology allows the same principle (ie in vitro amplification) to be applied to detect specific nucleic acid sequences. This method has many advantages.

Nowadays, PCR plays a central role in the genotyping and molecular epidemiology of organisms or individuals.

If you want to know more about PCR knowledge and news, please pay attention to the official website of generalbiosystems, here will update the knowledge and services about gene synthesis and PCR cloning technology in real time.